Phospho irf3 antibody
WebIRF3 Antibody detects endogenous levels of total IRF3 protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthtic peptide corresponding to a residues surrounding proline 307 of human IRF-3. Antibodies are purified by protein A and peptide affinity chromatography. WebFigure Legend Snippet: TAOK1 promotes TBK1-IRF3 complex formation. a HEK293T cells were transfected with plasmids encoding HA-TBK1, Flag-IRF3, and varying doses of a plasmid encoding Myc-TAOK1 (0.5, 1.0, and 1.5 μg). Cells were harvested 24 h after transfection for immunoblot analysis as indicated with the antibody to Flag tag. b …
Phospho irf3 antibody
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WebThe synthesized DNA sequence corresponding to the phospho-IRF3 (S386) monoclonal antibody was cloned into the plasmid and then transfected into the cell line for expression. The product was purified through the affinity-chromatography method and obtained the p-S386-IRF3 recombinant monoclonal antibody. WebPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation. The antibody was purified by affinity chromatography. Concentration. 0.5 mg/ml. Storage & Handling. The antibody solution should be stored undiluted between 2°C and 8°C. Application. WB - Quality tested.
WebPhosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity. Specificity This antibody recognizes IRF-3 phosphorylated at Ser396. Immunogen WebPhospho-IRF3 (Ser386) Antibody (PA5-78025) in WB. Western Blot using Phospho-IRF3 (Ser386) Polyclonal Antibody ( Product # PA5-78025 ). Untreated (–) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Phospho-IRF3 (Ser386) Polyclonal Antibody ( Product # PA5-78025) diluted …
WebWestern Blot analysis of Phospho-IRF3 (Ser386) in HeLa cells using a Phospho-IRF3 (Ser386) Polyclonal antibody ( Product # PA5-121307) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer 3% BSA was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. WebMouse liver lysates probed with Anti-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C. ... Rat Heart tissue lysates; Lane 4: Rat Testis tissue lysates probed with Phospho-IRF3 (Ser396) Polyclonal Antibody ...
Web中文名称： 磷酸化1型神经纤维瘤抗体 英文名称： phospho-NF1(Ser2817) 产品货号： bs-5521R 产品规格： 0.1ml 销售状态： 在售 磷酸化1型神经纤维瘤抗体双十一不但展开了五折促销活动，还上新了一批商品，公司建立以来，您的支撑与信赖能够给咱们带来更大
WebThis Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-IRF3 (S386) and total IRF3. An anti-pan IRF3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and IRF3 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. can a private company have stock amc theatersWebI haven't been able to detect human phospho-IRF3 (p-IRF3) on Wester Blot yet. The protocol I used: Block with blocking buffer for 1 hour. I tried blocking in 5% milk in TBST and in 0.5% BSA... fish feed pellet coolerWebBlot results show a ~50kDa IRF3 band that is detected by total IRF3 antibody (abcam 68481). None-specific bands occur for both total IRF3 and phospho IRF3 at 35 and 50kDa. I'm not sure... can a private homeschool have a gpaWebDuring infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and of a kinase complex that includes IKKε and TBK1 (6,7). IRF-3 is … can a private company take loan from llpWebMar 16, 2024 · Anti-Phospho-IRF3 (Ser386) Antibody (PACO24125) from Assay Genie is a high quality polyclonal antibody developed for research and validated in WB applications Toggle menu Select Currency: EUR fish feed market in indiaWebThe phospho-IRF3 (Ser386) assay measures IRF3 when phosphorylated at Ser386. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The phospho-IRF3 (Ser386) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. can a private company run a schoolWebAnti-IRF3 (phospho S386) antibody [EPR2346] (ab76493) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast … fish feed near me