WebThe phenol-sulfuric acid method was originally described as a nonspecific quantitative test for carbohydrates (Dubois et al. 1956). Later it was compared with the anthrone and N-ethylcarbazole methods (Handa 1966) and found to be best suited for total carbohydrate analysis because its ... WebMETHODS FOR ORGANIC CHEMICAL ANALYSIS OF MUNICIPAL AND ... METHOD 604—PHENOLS. 1. Scope and Application . 1.1. This method covers the determination of …
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WebThis method uses ultrafiltration (UF) in stirred cells followed by polysaccharide detection in the retentate by the phenol-sulfuric acid method. The UF method was compared with a conventional method based on ethanol extraction, dialysis, protein removal by trichloroacetic acid (TCA) and freeze-drying. WebA modified method is described to overcome this problem in which phenol was added after the dehydration of carbohydrates by sulfuric acid and after cooling the system. The color intensity around 475-485 nm for different compounds was fairly proportional to the amount of furfural derivatives (absorption at 310-320 nm) formed from the sugars in ... sharp calibration scanner
Method 604: Phenols - US EPA
WebSep 12, 2013 · The proposed method is a major improvement over the widely used Phenol–Sulfuric Acid method developed by DuBois, Gilles, Hamilton, Rebers, and Smith (1956). In the old method, furfural is allowed to develop color by reaction with phenol and its concentration is detected by visible light absorption. Here we present a method that … WebJul 21, 2016 · Under proper conditions, the phenol-sulfuric method is accurate to ± 2% (Nielson 2010). This seems to be real with the %CV shown in Tables 2 and 3 in the Absorbance columns. However, the accuracy of the outcomes is entirely based on the precision of the basic curve. WebIn the present work, the phenol-sulfuric acid method and the 3,5-dinitrosalicylic acid (DNS) method were developed with the aim to quantitatively analyze total sugars and reducing sugars, respectively. In regard with the phenol-sulfuric acid assay, 1.0 mL of sample was treated with 1.0 mL of 5% phenol, 5.0 mL of concentrated H 2 SO 4 porishima of triangle