WebThe GreenGate cloning method was employed to generate the complemented lines 73. To this end, the coding sequence of DEIP1 was amplified by PCR with primers cl_AT2G27290_For and cl_AT2G27290_Rev. ... WebGreenGate Cloning System to create plant expression vectors containing several cassettes and generate multi-construct transgenic plants - Jan Lohmann. MoClo Plant Parts II and Infrastructure Kit for hierarchical assembly of plant multigene constructs - …
Figure 1. The Golden Gate principle. A) Type IIS restriction...
WebOct 29, 2024 · Cloning Greengate entry module construction. Primers with GreenGate overhangs were designed following the Greengate protocol. Control primers were designed at the same time for colony screens and sequencing. For a list of primers see Supplemental Table S1. Construction of destination vectors with red membrane included Web57 rows · GreenGate Cloning System(Kit # 1000000036 ) Depositing Lab: Jan Lohmann. The 56 plasmid GreenGate cloning kit can be used to create plant expression vectors containing several cassettes and generate multi … how to scan from hp printer to dell laptop
Addgene: GreenGate - A Novel, Versatile, and Efficient Cloning …
WebThis plasmid is designed for Golden Gate cloning using the BsaI enzyme. These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were … Weband versatile cloning system for the generation of plant transfor-mation vectors, which we named GreenGate. The GreenGate system allows rapid and efficient assembly of six … WebGolden Gate cloning is one of the easiest cloning methods in terms of hands-on time, as digestion and ligation can be done in one 30-minute reaction. The destination vector and entry vector (s) are placed in a … north miami brewfest