WebApr 10, 2024 · Data were analyzed by GraphPad Prism software (v6) using unpaired, paired or one sample t-tests. The Aβ40 and Aβ42 levels were normalized against the corresponding GFP controls. The α-CTF levels were normalized to FL-APP and the corresponding GFP controls. All data are presented as mean ± SEM. Statistical … WebJul 19, 2024 · This way, CTF-GFP from APP-GFP variants were easily separated with the CTF-NT from APP-NT variants, and the effects of one of the two variants on another could be unambiguously demonstrated. Surprisingly, APP rIta -GFP significantly increased C99/C89 ratios of APP Wt -NT and APP rIta -NT.
PABPN1 suppresses TDP-43 toxicity in ALS disease models - PMC
WebAug 27, 2013 · Having established AP2 as a bridging molecule to mediate the interaction between LC3 and APP-CTF by IP, we next aimed to understand how the interaction of LC3 with AP2M1 and PICALM was affected by starvation-induced autophagy. HeLa cells were analyzed by IP experiments using anti-GFP antibody. WebCLU co-localizes with cytoplasmic TDP-43 inclusions in ER stressed cells and co-precipitates with TDP-43-GFP. a N2a cells transfected to express TDP-43 CTF-GFP and human CLU (hCLU) were treated as ... churchs on broadway tucson az
Adaptor complex AP2/PICALM, through interaction with LC3 ... - PNAS
WebJan 13, 2016 · To quantitatively determine the aggregation state of the molecules, we performed curve fitting for one- and two-component diffusion models of GFP and the … WebApr 13, 2024 · A C-terminal GFP tag and a C-terminal Flag tag were added to GPR111-CTF (or GPR111-CTF-ΔGPS) and GPR115-CTF (or GPR115-CTF-ΔGPS), respectively. All mutations were introduced using the QuikChange ... WebTo directly examine the effect of SUMOylation in CTF nuclear localization and apoptosis, we fused the SUMO moiety in-frame with GFP-CTF to form GFP-SUMO-CTF (Fig. 6A). Immunofluorescence studies indicated that GFP-SUMO-CTF resides in the nuclei of both U2OS ( p53 +/+ ) and Sa-OS2 ( p53 −/− ) cells ( Fig. 6 , A and B ). dew point of the gas