WebThere are two basic ways to visualize your RNA and DNA targets in situ—fluorescence (FISH) and chromogenic (CISH) detection. Characteristics inherent in each method of … WebThe CISH technique uses digoxigenin-labeled DNA probes that are specific for the HER2 gene locus on chromosome 17q11.2-21 to hybridize to the complementary nucleic acids present in the breast cancer specimen. The labeled HER2 probe is made using Subtraction Probe Technology (SPT™), a proprietary and patented technology

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Webspecific probe which targets the EBER transcripts in latently infected cells of the tissue sections. EBER ISH is routinely performed as a chromogenic ISH (CISH) whereby an … CISH probes are approximately 20 nucleotides in length and are designed for DNA targets. They are complementary to the targeted sequence and bind to it after a denaturation and hybridization step. Only a few CISH probes are available commercially, so for most applications they have to be extracted, … See more Chromogenic in situ hybridization (CISH) is a cytogenetic technique that combines the chromogenic signal detection method of immunohistochemistry (IHC) techniques with in situ hybridization. It was developed around the year … See more Compared to FISH FISH is considered to be the gold standard for the detection of chromosomal abnormalities because it is very sensitive and has high … See more Silver-enhanced in situ hybridization (SISH) SISH uses a similar method as CISH, but a silver precipitate is the end product rather than a brown product. In this method, a probe tagged with dinitrophenol (DNP) binds … See more Probe design Probe design for CISH is very similar to that for FISH with differences only in labelling and detection. FISH probes are generally labelled with a variety of different fluorescent tags and can only be detected under a … See more CISH is frequently applied to assess gene amplification, such as HER-2/neu status in breast cancer samples. HER-2/neu amplification is associated with higher mortality, higher … See more iranian air force insignia

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WebWe provide a wide variety of probes and kits aimed at the most predominant molecular slide-based tests performed in anatomic pathology. The IQFISH hybridization buffer is formamide-free and allows genomic DNA probe hybridization to be performed in Hybridizer outside a fume hood or in Dako Omnis in just 1-2 hours. WebRNA probes may refer to oligonucleotide probes composed of RNA, as well as labeled oligonucleotides that specifically target RNA transcripts. Such probes are used in fluorescent in situ hybridization (FISH) and chromogenic in situ hybridization (CISH) applications, which enable localization and measurement of specific DNA and RNA … WebCustom Probes Visualize the expression and spatial biology of target genes with ease AMPIVIEW™ RNA probes are uniquely designed with the precision of targeted, sequence specific RNA probes, powered by … iranian american journalist