Cdna synthesis supermix kit
WebqScript™ cDNA Synthesis Kit contains qScript™ Reverse Transcriptase, a mixture of a MMLV reverse transcriptase derivative and ribonuclease inhibitor protein, optimised for … WebReady-to-use supermix for unbiased, target-specific preamplification of cDNA or gDNA using up to 100 qPCR assays PCR Plastic Consumables A large selection of thin-wall …
Cdna synthesis supermix kit
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WebProduct Details The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. WebcDNA Synthesis • The LunaScript RT SuperMix can perform cDNA synthesis in the temperature range of 45°C to 65°C. 55°C is optimal for most applications. • In general, we recommend the transfer of 1 µl cDNA product into a 20 µl qPCR experiment. Where useful, up to 20% qPCR volume can
Web7 Jun 2024 · The first strand cDNA synthesis kits contain different reverse transcriptases with the major differences being their primary applications, format, and reaction temperature. Here is a summary of our three first strand cDNA synthesis kits. Links to this resource Related Products: ProtoScript® First Strand cDNA Synthesis Kit, WebThe iScript gDNA Clear cDNA Synthesis Kit integrates an effective DNase digestion step with fast and sensitive first-strand cDNA synthesis for accurate qPCR data. This kit …
WebThe kit offers two cDNA synthesis primers: Random Primers N6 and Oligo (dT)18, with the user's choice of Random as needed Primers N6, Oligo (dT)18 or Gene Specific Primers as reverse transcription primers, synthesized single-stranded cDNA The product can be used directly for subsequent PCR or qPCR reactions. Shipping and Storage WebmRNA can be easily purified using a polyA Spin™ mRNA Isolation Kit (NEB #S1560) or Magnetic mRNA Isolation ... In general, 1 ng to 1 μg total RNA or 0.1 ng to 100 ng mRNA are recommended. 2 cDNA Synthesis • LunaScript RT SuperMix can perform cDNA synthesis in the temperature range of 45°C to 65°C. 55°C is optimal for most …
Web13 rows · LunaScript ® RT SuperMix Kit: E3010S E3010L: Ideal for cDNA synthesis in a two-step RT-qPCR workflow; Single-tube supermix contains random hexamer and oligo …
WebDescription qScript cDNA SuperMix is a sensitive and easy-to-use 1-tube reagent for first-strand cDNA synthesis that combines a highly-modified RNAse H+ mutant of M-MLV … chief recovery officer city of houstonWebDescription. Hifair™ Ⅲ 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA Digester Plus) is a readjust premix developed based on Hifair™ⅢReverse Transcriptase. Compared with Hifair™ ⅡReverse Transcriptase, Hifair™ Ⅲ Reverse Transcriptase has significantly higher thermal stability and can withstand reaction temperatures up to 60 ... chief record nflhttp://biopioneer.com.tw/?p=41115 got aways at borderWebDesigned for efficient cDNA synthesis and amplification directly from intact single cells (1 to 1,000) or low amounts of total RNA (2 pg to 10 ng). Kit contains all required components … chief records officerWebCombined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than … got a whale of a tale to tell you ladsWeb25 Aug 2024 · The NAC (NAM, ATAF1/2, and CUC2) transcription factors are one of the largest families of transcription factors in plants and play an important role in plant development and the response to adversity. In this study, we cloned a new NAC gene, SlNAC10, from the halophyte Suaeda liaotungensis K. The gene has a total length of … chief recruitment officerWebThe SuperScript™ III First-Strand Synthesis SuperMix is an optimized SuperMix formulation for first-strand cDNA synthesis from purified poly(A) + or total RNA. RNA targets from 100 … Using the SuperScript™ III First-Strand Synthesis SuperMix The kit includes Sup… TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes chief recovery officer