WebExosomes from primarily cultured CAFs and paired normal fibroblasts (NFs) were collected and identified. The differential expression of exosomal miR-34c-5p between CAFs and NFs was detected by next-generation sequencing. In vitro and in vivo assays were performed to examine the effects of miR-34c-5p on the maintenance of stem-like properties. WebAug 5, 2024 · For comparison of three different groups (e.g., NFs, 4T1 CAFs, and CT26.WT CAFs), two-way ANOVA was used. Results Generic Isolation of Fibroblast Populations From Solid Tissues. The aim of the …
Frontiers Cancer-Associated Fibroblasts Promote …
WebDec 16, 2024 · CAFs and NFs were isolated and characterized as our previous work (Wang et al., 2024). Both CAFs and NFs displayed spindle-like shape, and morphology showed … WebFeb 4, 2024 · Cancer-associated fibroblasts (CAFs) are the main stromal components of cancer, representing a group of heterogeneous cells. Many studies indicate that CAFs promote tumor development. Besides, evidence of the tumor suppression effects of CAFs keeps on merging. In the tumor microenvironment, multiple stimuli can activate … microfiber drying towels amazon
Biological Characteristics and Genetic Heterogeneity …
WebNFs-exos and CAFs-exos by CRC cells (Additional file 1: Figure S3E, both P>0.05). In vitro and in vivo experiments were performed to evaluate the effect of NFs-CM, CAFs-CM, CAFs-exos or CAFs-CM depleted of exosomes treatment on CRC cells. Boyden chamber migration and matrigel invasion assay showed that both CAFs-CM and CAFs-exos … WebApr 7, 2011 · By using a study protocol approved by the Institutional Research Ethics Board, CAFs and NFs were cultured from 15 surgically resected primary NSCLCs, and the … WebMarker expression was confirmed by confocal microscopy comparing CAFs with primary NFs and commercial normal human lung fibroblast cells, WI-38 (Figure 1 B). Further, we analyzed CAF pro-tumor effects on NSCLC cell viability and migration in vitro. To this end, Calu-1 NSCLC cells were grown in conditioned medium (CM) derived from CAFs or NFs ... microfiber drying towel 39x59